用关键词“goblet cell quantification”检索，能检索到杯状细胞计数的文章：
2.5.1. Full CIC membrane quantification with cell counting software
A GC count for each CIC membrane was determined by summing the GC counts from all 25 images acquired with the aid of ImageJ (Schneider and Rasband, 2012) and the Cell Counter plugin (De Vos, 2010). The GCD was determined by dividing the GC count by 12 mm2 (25 images x 0.48 mm2 per image). This method served as the reference for comparison.
可见，用开源免费的ImageJ软件和Cell Counter插件进行杯状细胞计数已经有人做过了。ImageJ软件教程网上一抓一大把，例如利用ImageJ软件进行细胞/种子/颗粒计数。实在不行，直接人眼数数也行。
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